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magnetic cd19 microbeads  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec magnetic cd19 microbeads
    Expression of IA markers on B cells from BCPs versus HCs. PBMC from BCPs and HCs (2x10 6 /ml) were also stained to evaluate the expression of four markers of IA. (A) tSNE analyses of B cells (Live CD45+ <t>CD19+)</t> for the expression of the indicated IA markers, one representative individual/group. (B) Mean fluorescence intensity (MFI) for each marker. Results show mean MFI±SE from all individuals. (C) Combination gate analysis of four IA markers as shown by ring graphs. Each ring shows relative frequency of B cells expressing 1,2,3 or 4 markers simultaneously in each group. Mean comparisons between groups were performed by unpaired Student’s t test (two-tailed). *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.
    Magnetic Cd19 Microbeads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 620 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd19+microbeads/CD19+MicroBeads%2C+human/pmc13649394-47-8-11
    Average 96 stars, based on 620 article reviews
    magnetic cd19 microbeads - by Bioz Stars, 2026-10
    96/100 stars

    Images

    1) Product Images from "Phenotypic, functional and metabolic features of B cells from a small cohort of breast cancer patients"

    Article Title: Phenotypic, functional and metabolic features of B cells from a small cohort of breast cancer patients

    Journal: Frontiers in Oncology

    doi: 10.3389/fonc.2026.1944615

    Expression of IA markers on B cells from BCPs versus HCs. PBMC from BCPs and HCs (2x10 6 /ml) were also stained to evaluate the expression of four markers of IA. (A) tSNE analyses of B cells (Live CD45+ CD19+) for the expression of the indicated IA markers, one representative individual/group. (B) Mean fluorescence intensity (MFI) for each marker. Results show mean MFI±SE from all individuals. (C) Combination gate analysis of four IA markers as shown by ring graphs. Each ring shows relative frequency of B cells expressing 1,2,3 or 4 markers simultaneously in each group. Mean comparisons between groups were performed by unpaired Student’s t test (two-tailed). *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.
    Figure Legend Snippet: Expression of IA markers on B cells from BCPs versus HCs. PBMC from BCPs and HCs (2x10 6 /ml) were also stained to evaluate the expression of four markers of IA. (A) tSNE analyses of B cells (Live CD45+ CD19+) for the expression of the indicated IA markers, one representative individual/group. (B) Mean fluorescence intensity (MFI) for each marker. Results show mean MFI±SE from all individuals. (C) Combination gate analysis of four IA markers as shown by ring graphs. Each ring shows relative frequency of B cells expressing 1,2,3 or 4 markers simultaneously in each group. Mean comparisons between groups were performed by unpaired Student’s t test (two-tailed). *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.

    Techniques Used: Expressing, Staining, Fluorescence, Marker, Analysis, Two Tailed Test

    B cells from BCPs express higher levels of transcripts for NF-kB than those from HCs. PBMC were left unstimulated, then membrane stained with Live/Dead kit, anti-CD45 and anti-CD19 antibodies. (A) A representative experiment in which one HC and one BCP were evaluated. Negative control was the sample (HC) without the labeled probe. (B) Cumulative results from 10 BCPs and 10 HCs. Results show mean frequencies±SE. Mean comparisons between groups were performed by unpaired Student’s t test (two-tailed). ****p<0.0001.
    Figure Legend Snippet: B cells from BCPs express higher levels of transcripts for NF-kB than those from HCs. PBMC were left unstimulated, then membrane stained with Live/Dead kit, anti-CD45 and anti-CD19 antibodies. (A) A representative experiment in which one HC and one BCP were evaluated. Negative control was the sample (HC) without the labeled probe. (B) Cumulative results from 10 BCPs and 10 HCs. Results show mean frequencies±SE. Mean comparisons between groups were performed by unpaired Student’s t test (two-tailed). ****p<0.0001.

    Techniques Used: Membrane, Staining, Negative Control, Labeling, Two Tailed Test

    Related Articles

    other:

    Article Title: SPEN loss drives extra-follicular diffuse large B cell lymphoma with female-specific lethality and therapeutic vulnerabilities
    Article Snippet: SPEN loss drives extra-follicular diffuse large B cell lymphoma with 1 female-specific lethality and therapeutic vulnerabilities 2 3 Author List 4 Benedikt Pelzer1, Cem Meydan1,2, Isaac M Spiegel1, Ioannis Karagiannidis1, Min Xia1, Matt Teater1, Emma 5 M Welter3, Zowie E Searcy3, Laura K Hilton4, Darko Barisic1, Amos Fong4, Pengyan Fa1, Shenon Sethi5, 6 Irem S. Isgor5, Jessie J. Fielding6, Alireza Karbalayghareh7, Colin S. Burdette8, Sravya Tumuluru1, Sonia 7 M Debek1, Sunjae Lee1, Ramon Massoni-Badosa1,9,10, Ceyda Durmaz1, Eralda Salataj11, Prasath 8 Pararajalingam12, Zhengming Chen13, Richard J Pelzl1,14, Sanket Shah1, Martin A Rivas15, Kenneth B 9 Hoehn6,16, Coraline Mlynarczyk17, Hannah M Isles1, Xiang Wang18, Ahmet Dogan5, Kojo S. J. Elenitoba-10 Johnson5, David W Scott4, Kostiantyn Dreval19,20, Ryan D. Morin19, 20, 33, Christina S. Leslie7, Rishi Puri21, 11 Jacob B. Geri8,22, Christopher R Chin23-25, Amy Chadburn26, Christopher E Mason2,23,27, Hans Christian 12 Reinhardt28-31, Montserrat C Anguera3, Wendy Béguelin1,32*, Leandro Venturutti33-34**, Ari M Melnick1,35***.. 13 14 Affiliations 15 1.. Division of Hematology and Oncology, Department of Medicine, Weill Cornell Medicine, New York, NY, USA 16 2.

    Article Title: Enhancement of CD117-targeted bispecific T-cell engagement by CD33-targeted bispecific T-cell co-stimulation in acute myeloid leukemia
    Article Snippet: In selected cases, a CD3+/CD19+ double depletion was performed using human CD3 and CD19 MicroBeads (Miltenyi Biotec, cat# 130-136-718), following the manufacturer’s protocol.

    Article Title: Enhancement of CD117-Targeted Bispecific T-cell Engagement by CD33-Targeted Bispecific T-cell Costimulation in Acute Myeloid Leukemia
    Article Snippet: In selected cases, a CD3 + /CD19 + double depletion was performed using human CD3 and CD19 MicroBeads (Miltenyi Biotec, cat. #130-136-718), following the manufacturer’s protocol.

    Purification:

    Article Title: The dual role of peritoneal cavity B cells in the activation of antitumor T cells.
    Article Snippet: .. B cells from either the mouse SPL or PerC were purified using CD19 MicroBeads and an autoMACS Pro Separator (Miltenyi Biotec), according to the manufacturer’s instructions. ..

    Isolation:

    Article Title: IFN-γ-driven CD8 + T-cell-keratinocyte cross talk underlies inflammation and blistering in pemphigus lesions.
    Article Snippet: Journal Pre-proof IFN-γ–driven CD8+ T-cell–keratinocyte cross talk underlies inflammation and blistering in pemphigus lesions Yiting Shen, MD, Chuqiao Xu, MD, Qijun Wang, MD, Zixuan Huang, PhD, Huiyi Ke, MD, Wenqian Zhang, PhD, Tianyu Zhang, PhD, Yue Ruan, MD, Wenzhe Zhao, PhD, Hailun Wang, PhD, Jingying Wang, MD, Haiqin Zhu, PhD, Chuanxin Huang, PhD, Meng Pan, MD, PhD PII: S0091-6749(26)00330-1 DOI: https://doi.org/10.1016/j.jaci.2026.04.021 Reference: YMAI 17189 To appear in: The Journal of Allergy and Clinical Immunology Received Date: 24 November 2025 Revised Date: 2 April 2026 Accepted Date: 8 April 2026 Please cite this article as: Shen Y, Xu C, Wang Q, Huang Z, Ke H, Zhang W, Zhang T, Ruan Y, Zhao W, Wang H, Wang J, Zhu H, Huang C, Pan M, IFN-γ–driven CD8+ T-cell–keratinocyte cross talk underlies inflammation and blistering in pemphigus lesions, The Journal of Allergy and Clinical Immunology (2026), doi: https://doi.org/10.1016/j.jaci.2026.04.021.. This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.

    Article Title: A stromal platform for robust expansion of functional IL-10–producing B cells for immune regulation
    Article Snippet: Established lines were maintained in MEMα medium (Wako) supplemented with 10% (vol/vol) FBS (Biosera), 1% penicillin/streptomycin, and 2 mM L-glutamine. .. Primary human B cells were isolated from PBMCs by positive selection using CD19 MicroBeads (Miltenyi Biotec). .. In some experiments, enriched B cells were further purified by FACSMelody (BD Biosciences) cell sorter.

    Flow Cytometry:

    Article Title: IFN-γ-driven CD8 + T-cell-keratinocyte cross talk underlies inflammation and blistering in pemphigus lesions.
    Article Snippet: Journal Pre-proof IFN-γ–driven CD8+ T-cell–keratinocyte cross talk underlies inflammation and blistering in pemphigus lesions Yiting Shen, MD, Chuqiao Xu, MD, Qijun Wang, MD, Zixuan Huang, PhD, Huiyi Ke, MD, Wenqian Zhang, PhD, Tianyu Zhang, PhD, Yue Ruan, MD, Wenzhe Zhao, PhD, Hailun Wang, PhD, Jingying Wang, MD, Haiqin Zhu, PhD, Chuanxin Huang, PhD, Meng Pan, MD, PhD PII: S0091-6749(26)00330-1 DOI: https://doi.org/10.1016/j.jaci.2026.04.021 Reference: YMAI 17189 To appear in: The Journal of Allergy and Clinical Immunology Received Date: 24 November 2025 Revised Date: 2 April 2026 Accepted Date: 8 April 2026 Please cite this article as: Shen Y, Xu C, Wang Q, Huang Z, Ke H, Zhang W, Zhang T, Ruan Y, Zhao W, Wang H, Wang J, Zhu H, Huang C, Pan M, IFN-γ–driven CD8+ T-cell–keratinocyte cross talk underlies inflammation and blistering in pemphigus lesions, The Journal of Allergy and Clinical Immunology (2026), doi: https://doi.org/10.1016/j.jaci.2026.04.021.. This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.

    Magnetic Cell Separation:

    Article Title: NIK-driven IL-23 production by myeloid cells is a key factor in the development of autoimmune inflammation.
    Article Snippet: NIK (Map3k14) is a central regulator of noncanonical NF-κB signaling and immune homeostasis.. Mutations in this kinase are linked to autoimmune disorders, including multiple sclerosis (MS).. Both germline and T cell–specific deletion of NIK had been demonstrated previously to be associated with resistance to developing experimental autoimmune encephalomyelitis (EAE), an animal model for MS.

    Article Title: DNAM-1 immunoreceptor integrates innate and adaptive immune programs to drive intestinal inflammation
    Article Snippet: .. After washing, cells were incubated with CD11b, CD34, CD56, CD14, CD16, and CD19 microbeads together with anti-FITC microbeads (Miltenyi) for 15 min at 4°C, and unwanted cell populations were depleted using MACS. .. The enriched cell fraction was incubated with human FcR Blocking Reagent (Miltenyi) for 10 min on ice, followed by staining with the antibody cocktail for 60 min on ice.

    Incubation:

    Article Title: DNAM-1 immunoreceptor integrates innate and adaptive immune programs to drive intestinal inflammation
    Article Snippet: .. After washing, cells were incubated with CD11b, CD34, CD56, CD14, CD16, and CD19 microbeads together with anti-FITC microbeads (Miltenyi) for 15 min at 4°C, and unwanted cell populations were depleted using MACS. .. The enriched cell fraction was incubated with human FcR Blocking Reagent (Miltenyi) for 10 min on ice, followed by staining with the antibody cocktail for 60 min on ice.

    Selection:

    Article Title: A stromal platform for robust expansion of functional IL-10–producing B cells for immune regulation
    Article Snippet: Established lines were maintained in MEMα medium (Wako) supplemented with 10% (vol/vol) FBS (Biosera), 1% penicillin/streptomycin, and 2 mM L-glutamine. .. Primary human B cells were isolated from PBMCs by positive selection using CD19 MicroBeads (Miltenyi Biotec). .. In some experiments, enriched B cells were further purified by FACSMelody (BD Biosciences) cell sorter.



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    Expression of IA markers on B cells from BCPs versus HCs. PBMC from BCPs and HCs (2x10 6 /ml) were also stained to evaluate the expression of four markers of IA. (A) tSNE analyses of B cells (Live CD45+ <t>CD19+)</t> for the expression of the indicated IA markers, one representative individual/group. (B) Mean fluorescence intensity (MFI) for each marker. Results show mean MFI±SE from all individuals. (C) Combination gate analysis of four IA markers as shown by ring graphs. Each ring shows relative frequency of B cells expressing 1,2,3 or 4 markers simultaneously in each group. Mean comparisons between groups were performed by unpaired Student’s t test (two-tailed). *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.
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    Expression of IA markers on B cells from BCPs versus HCs. PBMC from BCPs and HCs (2x10 6 /ml) were also stained to evaluate the expression of four markers of IA. (A) tSNE analyses of B cells (Live CD45+ <t>CD19+)</t> for the expression of the indicated IA markers, one representative individual/group. (B) Mean fluorescence intensity (MFI) for each marker. Results show mean MFI±SE from all individuals. (C) Combination gate analysis of four IA markers as shown by ring graphs. Each ring shows relative frequency of B cells expressing 1,2,3 or 4 markers simultaneously in each group. Mean comparisons between groups were performed by unpaired Student’s t test (two-tailed). *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.
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    Image Search Results


    Expression of IA markers on B cells from BCPs versus HCs. PBMC from BCPs and HCs (2x10 6 /ml) were also stained to evaluate the expression of four markers of IA. (A) tSNE analyses of B cells (Live CD45+ CD19+) for the expression of the indicated IA markers, one representative individual/group. (B) Mean fluorescence intensity (MFI) for each marker. Results show mean MFI±SE from all individuals. (C) Combination gate analysis of four IA markers as shown by ring graphs. Each ring shows relative frequency of B cells expressing 1,2,3 or 4 markers simultaneously in each group. Mean comparisons between groups were performed by unpaired Student’s t test (two-tailed). *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.

    Journal: Frontiers in Oncology

    Article Title: Phenotypic, functional and metabolic features of B cells from a small cohort of breast cancer patients

    doi: 10.3389/fonc.2026.1944615

    Figure Lengend Snippet: Expression of IA markers on B cells from BCPs versus HCs. PBMC from BCPs and HCs (2x10 6 /ml) were also stained to evaluate the expression of four markers of IA. (A) tSNE analyses of B cells (Live CD45+ CD19+) for the expression of the indicated IA markers, one representative individual/group. (B) Mean fluorescence intensity (MFI) for each marker. Results show mean MFI±SE from all individuals. (C) Combination gate analysis of four IA markers as shown by ring graphs. Each ring shows relative frequency of B cells expressing 1,2,3 or 4 markers simultaneously in each group. Mean comparisons between groups were performed by unpaired Student’s t test (two-tailed). *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.

    Article Snippet: Total B cells were isolated from PBMC using magnetic CD19 Microbeads (Miltenyi 130-050-301), following the MiniMACS protocol (20 μL Microbeads + 80 μL PBS, for 10 7 cells).

    Techniques: Expressing, Staining, Fluorescence, Marker, Analysis, Two Tailed Test

    B cells from BCPs express higher levels of transcripts for NF-kB than those from HCs. PBMC were left unstimulated, then membrane stained with Live/Dead kit, anti-CD45 and anti-CD19 antibodies. (A) A representative experiment in which one HC and one BCP were evaluated. Negative control was the sample (HC) without the labeled probe. (B) Cumulative results from 10 BCPs and 10 HCs. Results show mean frequencies±SE. Mean comparisons between groups were performed by unpaired Student’s t test (two-tailed). ****p<0.0001.

    Journal: Frontiers in Oncology

    Article Title: Phenotypic, functional and metabolic features of B cells from a small cohort of breast cancer patients

    doi: 10.3389/fonc.2026.1944615

    Figure Lengend Snippet: B cells from BCPs express higher levels of transcripts for NF-kB than those from HCs. PBMC were left unstimulated, then membrane stained with Live/Dead kit, anti-CD45 and anti-CD19 antibodies. (A) A representative experiment in which one HC and one BCP were evaluated. Negative control was the sample (HC) without the labeled probe. (B) Cumulative results from 10 BCPs and 10 HCs. Results show mean frequencies±SE. Mean comparisons between groups were performed by unpaired Student’s t test (two-tailed). ****p<0.0001.

    Article Snippet: Total B cells were isolated from PBMC using magnetic CD19 Microbeads (Miltenyi 130-050-301), following the MiniMACS protocol (20 μL Microbeads + 80 μL PBS, for 10 7 cells).

    Techniques: Membrane, Staining, Negative Control, Labeling, Two Tailed Test